NEET : Multiple Choice Questions: DNA Finger Printing
1. Which of the following statement is not correct for the Repetitive DNA and Bulk DNA.
A. Small portion of DNA in which Sequences are repeated, called repetitive DNA.
B. The Repetitive DNA form 99.9 percentage of total DNA.
C. Bulk DNA codes all the protein for human.
D. Repetitive DNA does not code for any protein.
Answer: B Explanation
2. Consider the following statement and select the correct code:
1. The bulk DNA forms a major portion of genomic DNA.
2. Repetitive DNA form small portion of genome DNA hence called as satellite DNA.
A. Only 1.
B. Only 2
C.Both 1 and 2
D. Neither 1 nor 2
Answer: C Explanation
3. Satellite DNA is classified into micro, mini or macro satellite DNA on the basis of
A Composition of nitrogen bases
B Length of segment
C. Number of repetitive units
D. All of the above
Answer: D Explanation
4. Which one of the following statement is not correct.
A. Repetitive DNA is also called as satellite DNA on the basis of its length.
B. Satellite DNA is the base of DNA finger printing.
C. Bulk DNA is used in DNA finger printing due to its large proportion.
D. Repetitive DNA is different in person to person.
Answer: C Explanation
5. Repetitive DNA show high degree of polymorphism. Due to Polymorphism
A. Repetitive sequence form the basis of DNA fingerprinting.
B. Repetitive sequence are very useful identification tool in forensic applications.
C. Repetitive sequence are inheritable from parents to children.
D. All of the above.
Answer: D Explanation
6. DNA polymorphism refer to variation at genetic level due to
A. Recombination
B. Natural selection
C. Mutation
D . All of the above
Answer: C Explanation
7. DNA polymorphism can be observed if variation in allelic sequence has frequency
A. Equal to one
B. Greater than 0.01
C. Lower than 0.01
D. Greater than 1
Answer: B Explanation
8. Consider the following statement regarding the Variable number of tandem repeat ( VNTR)
1. These are small DNA sequences which are varied
from chromosome to chromosome in an individual.
2. The VNTR belongs to a class of satellite DNA referred to as mini-satellite.
3. VNTR show very high degree of polymorphism.
A. 1, 2 and 3 B. Only 3
C. 2 and 3 D. 1 and 3
Answer: A Explanation
9. The size of VNTR varies in size from
A. 0.1 to 20 kb
B. 30 to 40 kb
C. 50 to 100 kb
D. 40 to 100 kb
Answer: A Explanation
10. After hybridisation with VNTR probe gives many bands of differing sizes on
A. Ideogram
B. Chromatogram
C. Autoradiogram
D. Monogram
Answer: C Explanation
11. Consider the following statement and select the correct code
1. The bands of VNTR give a characteristic pattern of DNA and differs from individual to individual.
2. The bands of VNTR give a similar pattern of DNA in case of monozygotic twins.
12. Who of the following developed the technique of DNA finger printing by using satellite DNA.
A. Stanley Cohen
B. Howard Florey
C. Kary Mullis
D. Alec Jeffrey
Answer: D Explanation
13. DNA is isolated from blood, hair,semen ,skin etc through the
A. Electrophoresis
B. Centrifugation
C. Chromatography
D. Autoradiography
Answer: B Explanation
14. Multiple numbers of copies of isolated DNA are produced by
A. Polymerase chain reaction B. Gene cloning
C. Gene mapping
D. DNA replication
Answer: A Explanation
15. Amplification is
A. Cuting of DNA into fragments.
B. Sepration of DNA fragments
C. Formation of copies of DNA
D. Transfer of DNA into nylon plate
Answer: C Explanation
16. Consider the following statement of steps involved in finger printing and select the correct code
1.DNA is cut into fragments by using restriction enzymes.
2. DNA fragments are separated by gel electrophoresis .
3. Separated DNA fragment are visualised under ultraviolet radiation after the staining under electrophoresis.
16. Separated DNA fragment is transferred on the membrane of nitrocellulose or nylon membrane, called
A. Southern blotting
B. Northern blotting
C. Western blotting
D. Eastern blotting
Answer: A Explanation
17. Which of the following does not take place during hybridisation of finger printing.
A. Radioactive Probe is added with DNA fragment.
B. Probe form complementary strand by formation of hydrogen bond.
C. DNA fragment are kept under high temprature .
D. None of the above.
Answer: C Explanation
18. Which of the following statement is / are correct for Autoradiography.
A. The separated DNA fragments are exposed on X ray film.
B. An unique pattern of DNA is visualised on x-ray film in the form of Dark and light bands.
C.These unique bands are differ from person to person.
D. All of the above
Answer: D Explanation
19. Arrange the following steps in sequential order involved in DNA finger printing.
1. Hybridisation
2. Amplification
3. Southern blotting
4. Gel electrophoresis
5. Autoradiography
A. 1, 2, 3, 4, 5
B. 5, 4, 3, 2, 1
C. 2, 4, 3, 1, 5
D. 4, 2, 1, 3, 5
Answer: C Explanation
20. Which one of the following is/are application of DNA finger printing.
A. To identify the Criminal
B. To settle paternity dispute
C. To study the evolution
D. All of the above
Answer: D Explanation
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