Multiple ovulation embryo transfer

Multiple ovulation embryo transfer

• The term MOET stands for Multiple ovulation embryo transfer. This term was counted by Nicholas and Smith.

• This is an important technique for improving the herd of cattle.

• This technique involves two steps -

• First collection of fertilized eggs from genetically superior females and induced to produce a number of eggs.

• Second - The embryo is transferred into the reproductive tract of the desired female for achieving the goal of many progeny from a superior female.

• In Embryo transfer, newly formed embryos are removed from superior donor females and transferred into the reproductive tract of inferior females.

• In this technique, the embryo is also divided into two or more equal parts, each part is developed into an offspring having monozygotic twins. So embryo transfer technique is used for faster multiplication of superior cattle.

• The following steps are involved in embryo transfer-

Steps involved in Multiple ovule embryo transfer

• Step -1 A superior breed cow is selected as donor and a low milk producing cow is inferior.

• Step -2 Superovulation is induced in the donor by injecting follicle stimulating hormone.

• Step -3 The donor cow is either mated with an elite bull or inseminated artificially with semen of superior variety.

• Step -4 After seven day of  artificially insemination, the fertilized egg with eight to thirty two called stages are collected from the uterus of donor cow and transferred to the surrogate mother.

• Step -5 On an average, eighteen superior calves may be produced from one donor cow in a year.

• Step -6 Selected embryos are preserved in a special medium at 37 degree celsius.

• In order to freeze embryo for long term use, they are first kept in cryoprotectant after that they are transferred into liquid nitrogen at -196 degree Celeste. Such frozen embryo may be used after several years.

• This technique has been used for cattle rabbit mares etc.

• High milk yielding breeds of female and high quality meat yielding bulls have been breed successfully to increase the herd size in short time.